The bioaccumulation, elimination, and trophic transfer of BDE-47 in the aquatic food chain of Chlorella pyrenoidosa-Daphnia magna

Abstract

As a persistent organic pollutant, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) has been widely detected in aquatic environments. However, studies on the fate and transfer of BDE-47 in the aquatic food chain remain scarce. In this study, we investigated the bioaccumulation and elimination of BDE-47 in Chlorella pyrenoidosa, as well as the trophic transfer and biomagnification of BDE-47 in the “C. pyrenoidosa-Daphnia magna” food chain, using C-14 radioactive tracer technology. After 96 h of BDE-47 exposure, the algae accumulated 88.98% ± 0.59% of the initial radioactivity from the medium, and 36.09% ± 9.22% of the accumulated residues in the algae occurred in the form of bound residues. During 96 h of elimination, only 13% ± 0.50% of accumulated radioactivity in the algae was released into the medium. After 24 h of exposure, D. magna accumulated 35.99% ± 2.55% of the initial radioactivity via water filtration from the medium, and 31.35% ± 1.92% of the accumulated radioactivity in D. magna occurred as bound residues. However, D. magna accumulated 66.89% ± 2.37% of the accumulated radioactivity in the algae via food uptake from the contaminated algae, with a high portion of radioactivity observed as bound residues (83.40% ± 0.97% of accumulated radioactivity in D. magna). This indicated a reduction in the environmental risk of BDE-47. There was obvious biomagnification in the food chain between C. pyrenoidosa and D. magna (biomagnification factors, BMFs>1), resulting in environmental hazard transfer in the aquatic food chain. However, no metabolite was found during the exposure experiment, and further studies should be carried out to investigate the intrinsic mechanisms of the trophic transfer of BDE-47, especially in multilevel food chains. Therefore, this study elucidated the effect of dietary uptake on the bioaccumulation of BDE-47 in D. magna and provided new insight for future analysis regarding the bioaccumulation and biomagnification of organic pollutants in the food chain.