Abstract
Mesoporous silica xerogels with various amount of calcium (0, 5, 10 and 15%, named m-SXC0, m-SXC5, m-SXC10 and m-SXC15, respectively) were synthesized by template sol-gel methods, and cell responses to m-SXCs were studied using murine pre-osteoblast MC3T3-E1 in vitro. The results showed that cell morphology was not affected by m-SXCs indicating good biocompatibility. Furthermore, cell proliferation ratio on the m-SXCs increased over time, among which m-SXC10 was highest. NO production obviously rose with the increase of Ca content in m-SXCs. ALP activity and PGE(2) level on m-SXC5 significantly improved compared with m-SXC0 while decreased with the increase of Ca content for m-SXC10 and m-SXC15. No obvious discrepancy on osteopontin mRNA expressions was observed among m-SXCs. The collagen I and osteocalcin mRNA expression on m-SXC5 were up-regulated, while decreased on m-SXC15 evidently. The phosphorylation level of ERK 1/2 for the m-SXC10 was highest after 7 days. In conclusion, calcium in m-SXCs plays an important role in osteoblast activity, which indicates mesoporous silica xerogel containing appropriate calcium could stimulate osteoblast proliferation, differentiation, gene expression via the activation of ERK 1/2 signaling pathway, and shows great prospects in bone regeneration field using as a drug controlled release filler.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Materials Science: Materials in Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
