Abstract
The quantity of 3H-corticosterone bound to protein(s) in rat brain cytosol derived from animals subjected to controlled lighting was studied at 4-hr intervals throughout the day. The binding of ;3H-corticosterone to brain proteins was measured by gel chromatography on Sephadex G-25 and by precipitation of the proteins with protamine. In control (nonadrenalectomized, nonstressed) animals, the greatest amount of :3H-corticosterone binding occurred at 1200 and the lowest at 2000. The binding of 3Hcorticosterone was inversely related to the circulating amounts of endogenous corticosterone which indicated that the amount of endogenous steroid bound in brain appears to be related to the circulating blood levels. The results obtained with protamine and with the perfusion experiments demonstrate that the alterations in binding are not due to contamination of cytosol with CBG. When the hippocampus, septum and cerebral cortex from control animals were studied separately, the results were qualitatively the same as t...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
