The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Abstract

Diabetes not only increases the risk for cancer but also promotes cancer metastasis. Centrosome amplification (CA) is sufficient to initiate tumorigenesis and can enhance the invasion potential of cancer cells. We have reported that diabetes can induce CA, with diabetic pathophysiological factors as the triggers, which involves the signaling of nucleophosmin(NPM). Thus, CA can serve as a candidate biological link between diabetes and cancer. In the present study, we attempted to identify the NPMbinding partners and investigated whether the binding between NPMand its partner mediated the CA. We confirmed that high glucose, insulin, and palmitic acid cancer could elicit CA in the HCT16 colon cancer cells and found that the experimental treatment increased the binding between NPMand H2B, but not between p-NPMand H2B. The molecular docking analysis supported the fact that NPMand H2B could bind to each other through various amino acid residues. The treatment also increased the colocalization of NPMand H2B in the cytosol. Importantly, disruption of the NPM1-H2B complex by individualknockdown of the protein level of NPMor H2B led to the inhibition of the treatment-evoked CA. In conclusion, our results suggest that the binding between NPMand H2B proteins signals for the CA by high glucose, insulin, and palmitic acid.