The binary complex of pig plasma gelsolin with Mg 2+-G-actin in ATP and ADP

Abstract

Pig plasma gelsolin combined with Mg-G-actin at < 10 −8 M Cal 2+ to yield a binary complex. Complexes formed from G-actin with bound ATP or ADP. They contained approx. 1 mol of non-exchangeable nucleotide per mol of actin. ATP hydrolysis was not coupled to binary complex formation, but ATP in the complex hydrolysed very slowly. The nucleotide in the binary complex behaved like one of the two nucleotide molecules in the ternary complex (two actin monomers to one gelsolin), but the actin-gelsolin interaction was weaker in the binary complex.