Abstract
The importance of supplements used with exercise is increasing day by day. In this study, it was aimed to evaluate the effects of creatine monohydrate (CrM) supplementation with different intensities of exercise on oxidative stress through dynamic thiol disulfide homeostasis. Fourty two BALB/c mice were used and randomly divided into 6 groups; control (C), low-intensity exercise (LIE), high-intensity exercise (HIE), C+CrM (4% of daily diet), LIE+CrM, and HIE+CrM groups. Exercise groups were performed low-intensity (8m/min/30min/day) and high-intensity (24m/min/30min/day) exercise on a mouse treadmill for 8 weeks. At the end of the experimental period, the thiol disulfide homeostasis levels analyzed by using a new automated measurement technique. When the native thiol and total thiol values were examined the difference between the groups was statistically significant (respectively, p=0.029, p=0,035). Creatine intake with exercise decreased native thiol and total thiol levels. However, serum disulfide levels were lower in LIE+CrM compared to other study groups, but there was no statistically significant difference. It is thought that creatine supplementation with exercise reduces the thiol-disulfide homeostasis burden of the organism, and that after the depletion of creatine stores, the sustainability of oxidant-antioxidant homeostasis can be extended, thus prolonging the duration of antioxidant resistance.
Highlights
Creatine is an ergogenic molecule that contributes significantly to cellular energy metabolism
It is thought that creatine supplementation with exercise reduces the thiol-disulfide homeostasis burden of the organism, and that after the depletion of creatine stores, the sustainability of oxidant-antioxidant homeostasis can be extended, prolonging the duration of antioxidant resistance
We found that serum native thiol levels were statistically significantly higher in the low-intensity exercise (LIE) group (5765.0 ± 2372.4 μmol/L) and the high-intensity exercise (HIE) group (5819.1 ± 2063.4 μmol/L), compared to HIE+creatine monohydrate (CrM) group (2372.6 ± 876.0 μmol/L) (p 0.05)
Summary
Creatine is an ergogenic molecule that contributes significantly to cellular energy metabolism. It can be produced endogenously in body systems, or it can be taken into the body through diet. Cells with high energy requirements use creatine in the form of phosphocreatine (PCr) Havenetidis (2016). Skeletal muscle cells store enough ATP and phosphocreatine for approximately 10 seconds of high-intensity activity. Short-term creatine supplementation increases PCr stores by 10% to 40%, resulting in a 10% to 30% total creatine increase Kreider (2003), Momaya et al (2015). The use of creatine is limited to sports, but is a nutritional supplement with positive therapeutic effects in various diseases. It has been shown to prevent disease-related muscle atrophy, improve cognitive functions, and have
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