Abstract
The performance of two sets of primers targeting variable regions of the 16S rRNA gene V1–V3 and V4 was compared in their ability to describe changes of bacterial diversity and temporal turnover in full-scale activated sludge. Duplicate sets of high-throughput amplicon sequencing data of the two 16S rRNA regions shared a collection of core taxa that were observed across a series of twelve monthly samples, although the relative abundance of each taxon was substantially different between regions. A case in point was the changes in the relative abundance of filamentous bacteria Thiothrix, which caused a large effect on diversity indices, but only in the V1–V3 data set. Yet the relative abundance of Thiothrix in the amplicon sequencing data from both regions correlated with the estimation of its abundance determined using fluorescence in situ hybridization. In nonmetric multidimensional analysis samples were distributed along the first ordination axis according to the sequenced region rather than according to sample identities. The dynamics of microbial communities indicated that V1–V3 and the V4 regions of the 16S rRNA gene yielded comparable patterns of: 1) the changes occurring within the communities along fixed time intervals, 2) the slow turnover of activated sludge communities and 3) the rate of species replacement calculated from the taxa–time relationships. The temperature was the only operational variable that showed significant correlation with the composition of bacterial communities over time for the sets of data obtained with both pairs of primers. In conclusion, we show that despite the bias introduced by amplicon sequencing, the variable regions V1–V3 and V4 can be confidently used for the quantitative assessment of bacterial community dynamics, and provide a proper qualitative account of general taxa in the community, especially when the data are obtained over a convenient time window rather than at a single time point.
Highlights
The knowledge of the extent of bacterial diversity has expanded drastically since the introduction of culture-independent approaches based on molecular phylogenies of the small-subunit ribosomal RNA gene (16S rRNA gene)
Bacterial community profiles showed that both regions detected Proteobacteria as the dominant phylum, but differed in the relative abundance of Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria (Fig. 1)
Our results show that despite giving a biased estimation of bacterial diversity, different 16S rRNA gene primer sets used for high-throughput amplicon sequencing provide a similar quantitative measure of activated sludge bacterial population dynamics
Summary
The knowledge of the extent of bacterial diversity has expanded drastically since the introduction of culture-independent approaches based on molecular phylogenies of the small-subunit ribosomal RNA gene (16S rRNA gene). Recent studies have provided valuable insight into the bias introduced by primer selection for the estimation of diversity using generation sequencing in several ecosystems, such as soil [17], subgingival plaques [18], termite hindgut [19], human gut [20,21] and activated sludge (AS) [22,23]. These studies were primarily designed to determine which primer set could offer the most accurate taxonomic assignment of each microbiome. It is important to understand how microbial communities are structured in space [24] and time [25]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.