The better version of routine tissue processing technique-modified tissue processing

Abstract

We all know that “necessity is the mother of all inventions”, this applies very well to this technique. This experiment was carried out to overcome the drawbacks of routine tissue processing like difficult procurement, shrinkage, dull intensity of color, hardening etc. During routine tissue processing paraffin embedding is done before sectioning and the tissue has to undergo the process of fixation and dehydration in order to get prepared for sectioning. Very commonly used dehydrating agent which is considered to be a good one is ethyl alcohol but its purchase is subjected to many restrictions and causes shrinkage and hardening of tissue. Owing to this drawback of ethyl alcohol various other dehydrating agents were launched and used. We have tried a combination of isopropyl alcohol with acetone (3:1) and compared the section with routine tissue processing method. Sections prepared were stained with hematoxylin and eosin. The results obtained were much better than the routine technique when compared as well the modified technique was found to be cost effective as well. Keywords: Ethyl alcohol, Neutral Buffered formalin, Acetone, Isopropyl alcohol, Modified processing