The beneficial effect of natural antioxidants from olive oil with fig and date palm fruit extracts on biochemical and hematological parameters in rats during diethylnitrosamine-induced carcinogenesis

Abdallah H Fathy,Mohamed A Bashandy,Samir A Bashandy,Ahmed M Mansour,Peter Zahradka

doi:10.1139/facets-2017-0075

Abdallah H Fathy, Mohamed A Bashandy + Show 3 more

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https://doi.org/10.1139/facets-2017-0075

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Abstract

Diethylnitrosamine (DEN) is a well-known carcinogen. The aim of our study was to determine the role of olive oil (7 g/kg) with fig (1 g/kg) (OF) and (or) date palm (1 g/kg) (D) fruit extracts during DEN treatment of male Wistar rats. The OF–DEN and (or) D–DEN groups were given oral antioxidants daily for two weeks before and during DEN treatment (21 weeks).The DEN-treated group showed dramatic results for all investigated parameters as compared with the control rats. All OF–DEN and D–DEN groups showed significant improvement in hepatic thiobarbituric acid reactive substances, reduced glutathione, and nitric oxide concentration in the liver tissue, in addition to improvement in serum vascular endothelial growth factor level, alpha-fetoprotein, lipid profile, lipid risk ratios, and the hematological parameters as compared with the DEN-treated group.In conclusion, the administration of OF and (or) D fruit extracts to DEN-treated rats resulted in a considerable improvement in the investigated biochemical and hematological parameters. In addition, the combined OFD treatments showed greater improvements revealing the synergistic effect of the combination.

Highlights

Doxorubicin (DOX) is an anthracycline antineoplastic drug[1]
Each rabbit was treated with a single dose of DOX (Ebewe Pharma, Austria) (25mg/kg/i.p.) at the day 3; these animals were sacrificed at the day 5
The nephrotoxic effect of DOX characterized by decreasing glomerular filtration rate that leading to a rise in serum urea and creatinine

Summary

Introduction

Doxorubicin which is essential in treating breast and oesophageal carcinomas, solid tumors in childhood, osteosarcomas, Kaposi’s sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas[2]. Doxorubicin activation of stressrelated signaling pathways and cytokines receptors causes an imbalance between the pro-apoptotic and antiapoptotic factors; this imbalance will increase the permeability of mitochondrial membrane, enhance the release of cytochrome C oxidase, prolong the opening time of calcium channels in the sarcoplasmic reticulum, and end with cell death[9]. PTX had been reported to suppress the production of tumor necrosis factor-alpha (TNF-α) and modulate the production of other inflammatory cytokines[10]. It has the potential to decrease intraglomerular pressure and there was an early interest in pentoxifylline as a therapeutic agent in patients with kidney disease[13]

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