The behaviour of monoclonal antibodies in the First International Pig CD Workshop reacting with γδ/Null T lymphocytes in the blood of [formula omitted] line pigs

Abstract

Further studies were carried out on the monoclonal antibodies (mAbs) from the First International Swine CD Workshop which react with γδ Null T-lymphocytes, defined by the binding of mAb w020 141 (MAC320) as Swine Workshop Cluster number SWC6. Studies were also carried out on several other mAbs from the same workshop which identify other CD antigens, but whose binding is not restricted exclusively to γδ Null T-lymphocytes. The first group consists of 11 mAbs (w021, w022, w059–w065, w105 and w117) and the second group of 18 mAbs (w008, w026, w056, w067–w071, w080, w091–w094, w110, w111, w118, w119 and w121). All mAbs were characterised by binding to peripheral blood lymphocytes (PBL) from normal, sham-thymectomized (STx) and thymectomized (Tx) pigs of the Babraham SLAb b line and by their overlap, using two-colour immunofluorescence with biotinylated mAb MAC320 ( w020 141 , which identifies all γδ Null cell T-lymphocytes and the Null cell subpopulation identified by MAC319 (w021). The Null cell-specific mAbs were also used in inhibition studies of MAC319 and MAC320 binding and by staining PBL with pairs of mAbs together with either MAC319 or MAC320. Based on these data we suggest a putative relationship of the Null cell subsets defined by these mAbs with each other.