Abstract
The growth of carcinoma of the human large bowel was studied in the first 2 passages in immune deprived mice. The tumours were obtained from large bowel resections on 3 people. There was a strong histological similarity between the patient's tumour and the tumour that grew subcutaneously in the mice 2-8 months after implantation. One dissimilarity observed was a higher mitotic index in some of the tumours growing in the immune deprived animals. In the second passage of the bowel tumours, cells were implanted into groups of 8-10 animals in the following sites: subcutaneous, intramuscular, intravenous, intrahepatic, intraperitoneal and intrathoracic. Growth of tumour was observed from all 3 tumours when they were implanted subcutaneously, intramuscularly, intraperitoneally and intrathoracically. Infiltration of muscle by tumour was a frequent finding. Lung metastases developed after intravenous injection of cells in 1 of the 3 tumours. In none of the 3 tumours did growth follow injection of cells directly into the substance of the liver. On no occasions were spontaneous metastases observed.
Highlights
Summary.-The growth of carcinoma of the human large bowel was studied in the first 2 passages in immune deprived mice
CARCINOMA arising in the large bowel in man can be transplanted successfully subcutaneously in immune deprived animals (Povlsen and Rygaard, 1971; Castro, 1972; Cobb, 1972)
The work described in this article was designed to study the changes likely to occur when carcinoma of the large bowel is transplanted into immune deprived mice and, in addition, when this first passage tumour population is transplanted into a further group of immune deprived mice
Summary
Stock was obtained from the Laboratory had had intermittent diarrhoea for 6 months. Animals Centre, Carshalton, Surrey, England. The sex of the animals of the rectum was performed for carcinoma was chosen to match the sex of the patient. Animals and a higher dose of irradiation were used. Were obtained from the operating theatre and Irradiation was given at 60 rad/min using a implanted in the animals within 3 hours of. Without a bone marrow held at -190°C in 1000 dimethyl sulphoxide graft this dose of irradiation w%vould have been and 9000 Fischer's medium for months before lethal. Slices of tumour each approxmouse received 5 x 106 syngeneic femoral imately 3 x 3 x 1 mm were selected from bone marrow cells intravenously. The ani- different regions of each tumour and one mals received the tumour graft between 2 slice of tumour implanted subcutaneously and 4 -weeks after irradiation
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