Abstract
The approval of Genetically Modified Plants (GMPs) relies on the assumption that the inserts remain stable in the plant genome. Still, it has been shown that the integration of foreign DNA segments may coincide with minor or major target site re-arrangements and the occurrence of filler DNA segments. Little is known about the effect of conventional agricultural breeding or the influence of environmental conditions or physiological stresses on the integrity and stability of the insert, or the role of the insertion location. Our aim was to study putative sequence changes of a T-DNA insertion site in the genome of thale cress (Arabidopsis thaliana) under high light (photo-oxidative) stress, known to induce the formation of genotoxic Reactive Oxygen Species (ROS).
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