The basolateral amygdala to posterior insular cortex tract is necessary for social interaction with stressed juvenile rats

Abstract

Vocalizations, chemosignals, and behaviors are influenced by one’s internal affective state and are used by others to shape social behaviors. A network of interconnected brain structures, often called the social behavior network or social decision-making network, integrates these stimuli and coordinates social behaviors, and in-network connectivity deficits underlie several psychiatric disorders such as schizophrenia and autism spectrum disorders. Here, we investigated the role of the basolateral amygdala (BLA) and its projections to the posterior insular cortex, regions independently implicated in a range of sociocognitive processes, in a social affective preference (SAP) test. Viral vectors containing the gene coding for inhibitory chemogenetic receptors (AAV5-hSyn-hM4Di-mCherry) were injected into the BLA. SAP tests, which allow for the observation of unconditioned behavioral responses to the affective states of others, were conducted after inhibition of the BLA by systemic administration of the hM4Di agonist clozapine-n-oxide (CNO), or inhibition of BLA-insula terminals by direct infusion of CNO to the insula. After vehicle infusions, rats displayed preference for interactions with stressed juvenile conspecifics. However, CNO treatment eliminated preference behavior. The current results suggest that social decision making involves the transfer of emotional information from the BLA to the insula which represents a previously unrecognized anatomical substrate for social cognition.