The bacteriostatic effect of a new controlled-release carotenoids-silver nanoparticle

Abstract

Discuss the bacteriostatic effect of a novel controlled release silver nanoparticles carotenoids-silver nanoparticle. Detect the optimal extraction conditions for carotenoids through material liquid ratio, different ultrasonic temperatures and times; Detect the optimal synthesis conditions of carotenoids-silver nanoparticle through different volume ratios, UV irradiation times, and temperatures; Detect the bacteriostatic effects on Escherichia coli and Staphylococcus albus using the minimum inhibitory concentration (MIC) and Oxford cup agar plate diffusion method. The experiment proved that the best extraction effect of carotenoids was achieved when the volume ratio of orange peel powder to ethanol was 1:25, the ultrasound time was 40 min, and the temperature was 50 °C; When the amount of carotenoid extract added is 12 mL, the concentration of silver nitrate is 8 mmol/l, the UV irradiation time is 40 min, and the ultrasound temperature is 60 °C, the synthesized carotenoids-silver nanoparticle has the highest absorbance value; carotenoids-silver nanoparticle have strong inhibitory effects on the growth of both Escherichia coli and Staphylococcus albus. As the concentration increases, the bacteriostatic effect also becomes stronger, especially the strongest inhibitory effect on Staphylococcus albus. It was shown that carotenoids-silver nanoparticle has significant bacteriostatic effects, providing new ideas for the development of bacteriostatic agents in plant resources.