The Bacteriophage Pf-10-A Component of the Biopesticide "Multiphage" Used to Control Agricultural Crop Diseases Caused by Pseudomonas syringae.

Olesya A Kazantseva,Emilia I Kalamiyets,Tatsiana A Pilipchuk,Leonid N Valentovich,Andrey N Kazantsev,Andrey M Shadrin,Rustam M Buzikov

doi:10.3390/v14010042

Abstract

Phytopathogenic pseudomonads are widespread in the world and cause a wide range of plant diseases. In this work, we describe the Pseudomonas phage Pf-10, which is a part of the biopesticide “Multiphage” used for bacterial diseases of agricultural crops caused by Pseudomonas syringae. The Pf-10 chromosome is a dsDNA molecule with two direct terminal repeats (DTRs). The phage genomic DNA is 39,424 bp long with a GC-content of 56.5%. The Pf-10 phage uses a packaging mechanism based on T7-like short DTRs, and the length of each terminal repeat is 257 bp. Electron microscopic analysis has shown that phage Pf-10 has the podovirus morphotype. Phage Pf-10 is highly stable at pH values from 5 to 10 and temperatures from 4 to 60 °C and has a lytic activity against Pseudomonas strains. Phage Pf-10 is characterized by fast adsorption rate (80% of virions attach to the host cells in 10 min), but has a relatively small number of progeny (37 ± 8.5 phage particles per infected cell). According to the phylogenetic analysis, phage Pf-10 can be classified as a new phage species belonging to the genus Pifdecavirus, subfamily Studiervirinae, family Autographiviridae, order Caudovirales.

Highlights

Pseudomonas is a genus of Gram-negative bacteria including, inter alia, the known plant pathogen species
Phage Pf-10 was isolated in 2014 from a sample of green bean (Phaseolus vulgaris) tissue infected with P. syringae pv. syringae [40]
The Pf-10 phage was kindly provided by the Belarusian collection of non-pathogenic microorganisms (BIM) of the Institute of Microbiology, National Academy of Sciences, Minsk, Belarus

Summary

Introduction

Pseudomonas is a genus of Gram-negative bacteria including, inter alia, the known plant pathogen species. The phytopathogenic pseudomonads cause a wide range of plant diseases: necrotic lesions of fruit [1,2], stems [1,3], and leaves [1,2,4], hyperplasias (galls) [5], tissue macerations (rots) [6,7], cankers [1,8,9], blights [10], and vascular infections (wilts) [11]. Plant diseases caused by Pseudomonas are widespread throughout the world and infect a large number of higher plants, including agriculturally important crops, resulting in the loss of agribusiness profits and other consequential damages that have a negative impact on the world food supplies and economy.

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Conclusion