Abstract
16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea (SCS) were very diverse, which contained 22 of the 24 phyla of bacteria investigated from marine sediment, however, it was very imbalance among stations. So bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE (denature gradient gel electrophoresis) in this paper. The DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were significant. Thirty representative and differential fingerprints among samples were recovered and sequenced, phylogenetic analysis indicates that they may belong to Proteobacteria (α-, β-, γ-, δ-, ɛ-), Planctomycetes, Firmicutes, Chloroflexi, Acidobacteria, Actinobacteria, Nitrospirae, Gemmatimonadetes, candidate division WS3 and so on, of which, Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS sediment. This study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis.
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