Abstract
The bacterial composition in North Pacific Intermediate Water (NPIW) was investigated in three different years and compared with that in other seawaters around Japan. The results indicated that bacterial composition was surprisingly stable at the same point in a mesopelagic water mass throughout the years and supported previous physicochemical observations that NPIW is distributed to Kumejima, Japan.
Highlights
The bacterial composition in North Pacific Intermediate Water (NPIW) was investigated in three different years and compared with that in other seawaters around Japan
Based on the percent OTU composition profiles of waters, a nonmetric multidimensional scaling (NMDS) plot based on Bray-Curtis similarity was constructed using the PAST software
15,520 a The original water masses were deduced from the description by Taniguchi [3] and from temperature-salinity data, close to the sampling locations, from Argo JAMSTEC
Summary
The bacterial composition in North Pacific Intermediate Water (NPIW) was investigated in three different years and compared with that in other seawaters around Japan. DNA extraction, PCR amplification of the V1 to V3 region of the 16S rRNA bacterial gene, emulsion-based clonal amplification using a Lib-A kit (Roche), and sequencing on the GS Junior 454 system (Roche) were conducted as described previously [4]. Tag and primer regions used for PCR were removed from the sequences by QIIME (http:// qiime.org/) [5] and manually in Se-Al after alignment in ClustalX (version 2.1) [6].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
