Abstract
Abstract Phytohormones occur in bound and free forms. The bound form is designated by some as conjugated if the hormone is covalently linked to a small molecule and as bound if it is attached to a macromolecule or cell particle (39). However, the terms bound and conjugated will be used interchangeably here, as they are in many other treatises. There is more information published on the relationship between free and conjugated 1H-indole-3-acetic acid (IAA) than is available on similar relationships of other hormones. Free IAA is the active form of the hormone. This activity was seen, for example, when IAA conjugates were applied to the first internode section of bean (Phaseolus vulgaris L.). The promotion of curvature was traceable to the amount of free IAA released from the conjugates (4). Excellent reviews of conjugated auxins are available (3, 9). It is the purpose of this communication to acquaint the reader with some of the broader aspects of conjugated auxin and then to focus on the use of auxin conjugates in tissue culture.
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