The Autowave Mode of the Formation of Dinitrosyl Iron Complexes with Thiol-Containing Ligands

Abstract

Addition of a 20-μL drop of a mixture containing 1 mM Fe2+ + 0.5 M glutathione to the surface of 0.5 M S-nitrosoglutathione solution, which is expected to form dinitrosyl iron complexes with glutathione, generated short-lived (0.2 s) concentric ring-shaped structures in the S-nitrosoglutathione solution. The number of the ring-like structures exceeded the number of concentric circles spread across the water surface (mechanical waves) when droplets of water fall into a body of an aqueous solution. It appears likely that the ring-shaped structures observed in the S-nitrosoglutathione solution are associated with an autowave distribution of products generated during the formation of dinitrosyl iron complexes with glutathione.