Abstract
The components and operation of an automatic system for the assay and data compilation of enzyme reaction rates is described. Programs suitable for calculation of a large number of single and differential, as well as average and maximal, linear rates by the method of least squares are presented. Sample assays by manual and computer techniques are compared for the enzymes tyrosine α-ketoglutarate transaminase, glucose-6-phosphate dehydrogenase:6-phosphogluconate dehydrogenase and glucokinase-hexokinase. Good agreement is obtained between the two methods. Possible other uses of the instrument are discussed.
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