The Australian lungfish (Neoceratodus forsteri) - fish or amphibian pattern of muscle development?

Abstract

The Australian lungfish Neoceratodus forsteri (Dipnoi-Sarcoterygians) is a likely candidate for the extant sister group of Tetrapoda. Transmission electron and light microscopy analysis revealed that the arrangement of somite cells of the lungfish resembles the structure of the urodelan somite. On the other hand, the pattern of early muscle formation in N. forsteri is similar to that found in the Siberian sturgeon (Acipenser baeri). During the early stages of myogenesis of N. forsteri, somite-derived cells fuse to form multinucleated muscle lamellae. During later stages, mononucleated undifferentiated cells are first observed in the intermyotomal fissures and subsequently in the myotomes, among white muscle lamellae. The cells from the intermyotomal fissure differentiate into fibroblasts. The cells which have migrated into the myotomes, differentiate into mesenchyme-derived myoblasts. After hatching, white muscle lamellae are successively converted into polygonal muscle fibres. Conversion of lamellae into fibres may occur through splitting of muscle lamellae, or cylindrical muscle fibres may arise de novo as a result of fusion of mesenchyme-derived myoblasts. No increase in the number of muscle fibre nuclei is observed either in embryonic or juvenile musculature of N. forsteri. We suggest that until the 53 stage of embryonic development, the increase in muscle mass is accomplished mainly through hyperplasy. Thus, lungfish muscle represents the organizational intermediate between fishes and amphibians. This makes it a useful model to study the evolutionary implications of the mechanisms of muscle development.