The attempt of human amniotic fluid colony-derived stem cells for cell therapy in animal model of ventricle injury

Abstract

Objective To investigate the potential of human amniotic fluid colony-derived stem cells (hAFCSCs) transplanted into lateral ventricle injury in rats for cell therapy. Methods The second-trimester amniotic fluid samples were collected through amniocentesis by ultrasound from gestational age of 16 to 22 weeks for routine prenatal diagnosis purposes and samples were only used when cytogenetic analysis revealed no major abnormalities. Using colony poculum, hAFCSCs were isolated from second-trimester amniotic fluid. The ventricle damage model is divided into normal control group, control group and experimental group, blank control group. The hAFCSCs were injected into the ventricle of the animal model. To analyse the growth (weight, brain weight, open ears, grow hair) and sensorimotor function (foot test, reflection, limb position) change in model at several points. All statistic analysis of the data was performed with SNK to compare the difference of these groups. The protein of Nestin was detected using West blotting in the brain tissue of mode. Results There were no significant differences in body weight and brain weight of rats in each group. There was no remarkable difference in open ears between the experimental group, the control group and the blank control group, but there were notable differences between the mentioned groups and the control group. There were differences in sensorimotor function between experimental control group, blank control group and the normal control group, the experimental group; the experimental group were close to that of the normal control group. It showed that the expression of nestin after 5 days cells injection with Western blotting. Conclusion The hAFCSCs could improve the growth, sensorimotor function of the ventricle damage model rat. Key words: Human amniotic fluid colony-derived stem cells; Lateral ventricle injury; Animal model; Cell therapy