The Associations of Periodontopathic Bacteria and Oral Candida with Periodontal Inflamed Surface Area in Older Adults Receiving Supportive Periodontal Therapy.

Hideo Shigeishi,Masaru Sugiyama,Yoshino Kaneyasu,Momoko Ishikawa,Iori Oka,Cheng-Yih Su,Mariko Nakamura,Kouji Ohta,Kanako Yano

doi:10.3390/diagnostics11081397

Hideo Shigeishi, Masaru Sugiyama + Show 7 more

Open Access

https://doi.org/10.3390/diagnostics11081397

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Journal: Diagnostics (Basel, Switzerland)	Publication Date: Aug 2, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Hiroshima University

Abstract

The periodontal inflamed surface area (PISA) has been proposed for assessment of the total periodontal inflammatory status in people with periodontitis. This study was performed to investigate the associations of periodontopathic bacteria and candida with PISA in older people. We enrolled 100 patients aged ≥ 60 years who visited Hiroshima University Hospital. PISA and periodontal epithelial surface area (PESA) were calculated in each patient. Oral rinse samples were collected for DNA extraction. Periodontopathic bacteria and candida were detected by polymerase chain reaction. The mean values of PISA and PESA were significantly greater in T. forsythia-positive patients than in T. forsythia-negative patients. T. forsythia/C. albicans double-positive patients exhibited significantly greater PISA values than did non-double-positive patients. Additionally, PISA values were significantly greater in T. forsythia//T. denticola/C. albicans triple-positive patients than in T. forsythia//T. denticola/C. albicans non-triple-positive patients (p = 0.02). Propensity score-matching was performed between periodontopathic bacteria-positive and -negative patients using propensity scores generated from clinical factors. Importantly, T. forsythia/T. denticola double-positive patients exhibited significantly greater PISA values than non-double-positive patients among 72 propensity score-matched patients. Our preliminary results highlight the importance of the presence of T. forsythia and T. denticola for periodontal inflammation severity in older Japanese people.

Highlights

A dynamic balance between oral microorganisms and the host is critical for the maintenance of oral health [1]
-called red complex bacteria such as Treponema denticola, Tannerella forsythia, and Porphyromonas gingivalis are associated with severe periodontitis [3]
The objective of this study was to investigate the associations of periodontopathic bacteria (e.g., P. gingivalis, T. forsythia, and T. denticola) and Candida albicans with periodontal inflamed surface area (PISA) in older people

Summary

Introduction

A dynamic balance between oral microorganisms and the host (involving genetics, host immune response, and circadian rhythm) is critical for the maintenance of oral health [1]. -called red complex bacteria such as Treponema denticola, Tannerella forsythia, and Porphyromonas gingivalis are associated with severe periodontitis [3]. Both P. gingivalis and T. denticola are present in subgingival plaque at deep periodontal pockets [4]. Microbial colonization with red complex bacteria is preceded by colonization with so-called orange complex bacteria (i.e., Fusobacterium subspecies, Prevotella intermedia, Prevotella nigrescens, Peptostreptococcus micros, Campylobacter showae, Campylobacter gracilis, Campylobacter rectus, Streptococcus constellatus, and Eubacterium nodatum) [3] These red and orange complex bacteria have predominant roles in the etiology of periodontitis. Both periodontopathic bacteria and oral candida are presumed to initiate active inflammation in periodontal tissues

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