The associations of indices of echocardiography with polymorphism of genes of angiotensinogen and angiotensin receptor type I in examined patients with chronic rheumatic heart disease

Abstract

The purpose of study is to evaluate associations of polymorphism of genes encoding components of renin-angiotensin system and indices of echocardiography in examined patients with rheumatic heart disease (RHD). The sampling consisted of 128 patients with RHD and average age 58,96±0,34 years. The echocardiography was implemented using Philips Affinity 50 machine. The genetic typing was carried out according polymorphic markers Thr174Met, Met235Thr and А1166С by polymerase chain reaction in real-time with electrophoretic scheme of detecting the result of "SNP-EXPRESS". The heterozygosis of Thr174Met results in larger dilatation of left sections of heart. The Thr174Thr homozygotes characterized by large linear dimensions of right heart. The Thr235Thr homozygotes characterized by minimal parameters reflecting hypertrophy of left ventricle of heart. There was no significant difference in echocardiography indices in patients with A1166A polymorphism. The mutations in codon 235 and 174 did not affect distance of 6-minute walk test. The statistically significant difference in the distance of 6-minute walk test was obtained only for А1166С: the minimum distance indicators is in the group of A1166C heterozygotes - 291,46 (273,83-309,09) meters and the maximum distance in С1166С homozygotes - 357,20 (309,21-405,19) meters. In patients with RHD, Thr174Met heterozygosis can lead to dilatation of left heart camera, while homozygosis for Thr174Mr leads to dilatation of right heart camera and left ventricular hypertrophy. The Met235Thr heterozygosis, on the contrary, are characterized by minimal sizes of left atrium and right ventricle. Polymorphism of the A1166C gene encoding angiotensin type I receptor had practically no effect on echocardiography indices in patients with RHD.