The association of HpDof1.7 and HpDof5.4 with soluble sugar accumulation in pitaya fruit by transcriptionally activating sugar metabolic genes

Abstract

Abstract Soluble sugar is one of the most important factors affecting fruit flavor and quality. Here, we report the identification of two Dof (DNA-binding with one finger) transcription factors termed HpDof1.7 and HpDof5.4 and their roles in influencing sugar accumulation in pitayas. HpDof1.7 and HpDof5.4 share a similar expression pattern with sugar metabolism-related genes HpSuSy1 and HpINV2, and sugar transporter genes HpTMT2 and HpSWEET14 during pitayas maturation, and their expression pattern was also consistent with the accumulation of glucose and fructose, which were the predominant sugars in pitayas. HpDof1.7 and HpDof5.4 were both typical nucleus-localized proteins with trans-activation ability. Gel mobility shift assay revealed that HpDof1.7 and HpDof5.4 were bound to promoters of HpSuSy1, HpINV2, HpTMT2 and HpSWEET14. Finally, transient expression assays in tobacco leaves showed that HpDof1.7 and HpDof5.4 increased the activities of HpSuSy1, HpINV2, HpTMT2 and HpSWEET14 promoters, thus facilitating sugar accumulation by transcriptionally enhancing sugar metabolic pathway genes. Our findings provide a new perspective on the regulatory mechanisms of Dof transcription factors in sugar accumulation and pitaya fruit quality formation.