The Association of Fat-Mass-and Obesity-Associated Gene Polymorphism (rs9939609) With Colorectal Cancer: A Case-Control Study.

Maryam Gholamalizadeh,Samira Rastgoo,Hossein Sadeghi,Saeid Doaei,Zahra Aslany,Elham Kheyrani,Alireza Mosavi Jarrahi,Mohammad Esmail Akbari,Melika Razavi Hashemi,Sayed Hossein Davoodi,Ghasem Azizi Tabesh,Azadeh Hajipour,Bojlul Bahar,Reza Mirfakhraie

doi:10.3389/fonc.2021.732515

Abstract

Background and AimThe association between the rs9939609 polymorphism of fat mass and obesity-associated gene (FTO) and risk of colorectal cancer is controversial. This study aims to evaluate the relationship between FTO rs9939609 polymorphism and colorectal cancer (CRC) in Iranian people.MethodsA case-control study was conducted on 125 patients with CRC and 250 healthy subjects in Tehran, Iran. Demographic data and blood samples were collected from all participants. Genotyping of rs9939609 polymorphism was performed by the tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) method.ResultsThe occurrence of AA genotype of FTO rs9939609 polymorphism in the colorectal cancer patients was significantly higher compared to that of healthy subjects (16.4 vs. 2.9%, respectively, P=0.02). The association between the frequency of risk allele of the FTO polymorphism and CRC (B=1.67, P=0.042) remained significant after adjustment for age. Further adjustment for gender (model 2) and marital status (model 3) did not change this result (B=1.67, P= 0.042 and B=1.67, P=0.043, respectively). The results remained significant after additional adjustment for ethnicity (B=1.57, P= 0.047).ConclusionWe found a positive association between the A allele of the rs9939609 polymorphism and CRC. Future studies are required to identify the underlying mechanisms.

Highlights

Colorectal cancer (CRC), known as bowel cancer, colon cancer, rectal cancer, is characterized by the abnormal growth of tissue in the large intestine leading to cancer [1]
We found a positive association between the A allele of the rs9939609 polymorphism and CRC
Based on the fact that the fat mass and obesity-associated gene (FTO) rs9939609 variants have shown an association with obesity in various ethnicity and obesity is a risk factor for CRC aetiology, the objective of this study was to investigate the genetic association of FTO rs9939609 variants with the risk of developing CRC in Iranians

Summary

Introduction

Colorectal cancer (CRC), known as bowel cancer, colon cancer, rectal cancer, is characterized by the abnormal growth of tissue in the large intestine leading to cancer [1]. With 1–2 million new cases and 700,000 deaths every year, CRC is one of the most common cancers worldwide [2]. CRC is among the top three cancers most diagnosed, with an incidence rate of 11% of all cancers. While the incidence of CRC is high in developed nations, there are rapid increases in cases in developed countries, including Iran. Based on a screening program completed in 2018, 33 Iranians per 100,000 have CRC [4]. There has been a year-to-year increase in the overall risk of developing this cancer among the Iranians [5]. The association between the rs9939609 polymorphism of fat mass and obesity-associated gene (FTO) and risk of colorectal cancer is controversial. This study aims to evaluate the relationship between FTO rs9939609 polymorphism and colorectal cancer (CRC) in Iranian people

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Discussion

Conclusion