The association of factor V Leiden mutation (G1691A) with pregnancy complications (miscarriage) in the Iran, East Azerbaijan

Abstract

Objective: The pathogenesis of human spontaneous abortion involves a complex interaction of several genetic and environmental factors. Resistance to anticoagulant activity of activated protein C (APC), often due to point mutations in Factor V gene. Leiden mutation is definitely associated with pregnancy complications. In this study, we have investigated association of factor V Leiden with recurrent miscarriage in Iranian patients. Methods: 200 women included in this study: 100 women as patients group with 2 or more consecutive unexplained miscarriage and 100 women as controls group with at least one childbirth and without any abortion or pregnancy complications. Genomic DNA extracted from peripheral blood of each person. Presence or absence of mutations in the factor V Leiden gene performed by factor V Leiden coagulation test, and to determine homozygous and heterozygous of the factor V Leiden mutation, used HRM techniques by PCR Factor V Leiden Kit. Results: In this study, Chi-square analysis was significant relationship between mutant G1691A and recurrent pregnancy loss, and other environmental variables in the statistical analysis, gestational age and family history, statistically significant association with recurrent pregnancy loss. Conclusions: The results of this study showed that Factor V Leiden mutation has an effective role in the risk of pregnancy complications and recurrent miscarriage. Factor V Leiden mutation frequency in various countries due to genetic differences and different geographic can prone to recurrent pregnancy loss, better management of patients and adoption of effective preventive methods.