The Association of Antioxidants Gene Polymorphisms (SOD2 Ala16Val, GPx1 Pro198Leu, GSTP1 Ile105Val, and Cat −21 A/T) and Risk of Type 2 Diabetes Mellitus

Abstract

BACKGROUND: Antioxidant gene polymorphism is one of the genetic risk factors associated with type 2 diabetes mellitus (T2DM) incidence.
 AIM: This study was to analyze the association of superoxide dismutase 2 (SOD2) Ala16VAl, glutathione peroxidase (GPx1) Pro198Leu, glutathione S-transferase Pi1 (GSTP1) Ile105Val, and Cat −21 A/T gene polymorphisms and risk of T2DM.
 METHODS: We genotyped deoxyribonucleic acid of 120 T2DM patients and 80 healthy control by polymerase chain reaction and restriction fragment length polymorphism method, using a specific restriction enzyme.
 RESULTS: This study showed that the Val/Val of SOD2 was significantly associated with an increased risk of T2DM compared to the Ala/Ala+Ala/Val (p = 0.011; odds ratio [OR] = 2.220; confidence interval [CI] = 1.234–3.992). The TT genotype of Cat gene was also significantly associated with an increased risk of T2DM compared to the AA genotype (p = 0.027; OR = 5.000; CI = 1.079–23.176) and TT genotype to the AA+AT genotype (p = 0,030; OR = 4.738; CI = 1.039–21.600). However, there was no difference in all genetic models of GPx1 Pro198Leu and GSTP1 Ile105Val gene polymorphisms (p > 0.05).
 CONCLUSION: This study indicates that the Val/Val under the recessive model of SOD2 gene also TT genotype under the co-dominant model of Cat gene and TT genotype under the recessive model of Cat gene were associated with risk factors for T2DM occurrence.