The Association Between Interleukin-10 Gene Polymorphism And Il-10 Levels In Geohelminth Positive Adolescents And Adults In West Sumatera

Abstract

Geohelminth infection can cause nutritional disorder and anemia. Behind these negative effect, worms turned out to provide protective effect on a number of diseases such as allergies and inflammations. This is related to its ability to modulate the host's immune response via Th-2, characterized by an increase production of Interleukin 10 (IL-10) which is an anti inflammatory cytokine. This study aimed to investigate the IL-10 polymorphisms in geohelminth infections and control and assess their effects on IL-10 production. This research was a comparative cross-sectional study. IL-10 levels were measured by using the ELISA method. IL-10 gene polymorphism was examined by using PCR and the Sanger sequencing method to detect SNP at rs1800896, rs1800871 and rs1800872. All subjects infected with geohelminth in this study were classified as mild infections. There was a significant increase in IL-10 levels in geohelminth-infected individuals when compared to controls (p 0.05), but there was a significant association between GA genotype in rs1800896 with elevated levels of IL-10 in geohelminth infections (p <0.05). We concluded that high levels of IL-10 in geohelminth infections were also influenced by IL-10 gene polymorphism, specially the GA genotype at rs1800896