Abstract
Expression of proinflammatory cytokines, such as interleukin (IL)-6 (IL-6) and metalloproteases, are elevated in patients with rotator cuff tear (RCT). In order to investigate the role of IL-6 gene polymorphisms on RCT risk, we genotyped two SNPs on IL-6 gene (rs1800795 and rs1800797) in 138 RCT patients and 137 healthy controls using polymerase chain reaction (PCR) and Sanger sequencing. The IL-6 expression in shoulder joint synovial fluid was determined by using enzyme-linked immunosorbent assay (ELISA) method. The constant score and visual analog scale (VAS) were used to evaluate the clinical outcome of two s (surgicsal vs. conservative) for RCT patients. For rs1800795, individuals with the GG genotype or G allele had significantly higher risk of RCT. Elevated risk of tear size was associated with the GG genotype of the rs1800795 polymorphism. The IL-6 rs1800797 polymorphism was also associated with an increased risk of RCT, especially among female, drinkers, and individuals with B(MI) < 25 kg/m2. The elevated levels of IL-6 gene were observed among the mutant genotype of rs1800795/rs1800797 polymorphism. Surgical group is significantly better than conservative treatment from the perspective of constant score and VAS. Furthermore, CG genotype of rs1800795 polymorphism increased the constant score at 6 months in comparison with CC genotype. In conclusion, our study supports a role of IL-6 rs1800795/rs1800797 polymorphisms on increased RCT risk. The RCT patients with CG genotype of rs1800795 polymorphism have more obvious surgical treatment effects by influencing the IL-6 expression.
Highlights
Rotator cuff disease is the most common etiology of shoulder pain, which leads to discomfort, pain and motor dysfunction [1]
We recorded family history of Rotator cuff tear (RCT) and tear size of RCT patients to investigate its association with IL-6 rs1800795/rs1800797 polymorphisms (Table 1)
No significant deviation from Hardy–Weinberg equilibrium (HWE) was found for rs1800795/rs1800797 polymorphisms in both cases and controls, suggesting these subjects are representative of the local population
Summary
Rotator cuff disease is the most common etiology of shoulder pain, which leads to discomfort, pain and motor dysfunction [1]. Rotator cuff tear (RCT) is present in 20–54% of persons aged between 60 and 80 years [2,3]. The prevalence of RCTs increases significantly [4]. Patients with full-thickness RCT have increased levels of synovial inflammation in comparison with healthy controls [7]. In patients with RCT, expression of IL-1, IL-6 and TNF-α in the subacromial bursa has been shown to be elevated in comparison with healthy controls [9]. Higher IL-1β and IL-6 levels were related to more severe pain in animal RCT models [10]. IL-6 expression levels are higher in ruptured Achilles tendon compared with normal Achilles tendon and painful Achilles tendon [11]
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