The Association Between CYP24A1 rs4809957 Gene Polymorphism and Grade of Colorectal Cancer at Prof. Dr. I G.N.G Ngoerah Hospital in Bali

Abstract

Introduction: Colorectal cancer (CRC) is a malignancy in the large intestine, including the colon and rectum. One of the pathogenesis of CRC is the molecular pathway. This study aimed to evaluate the association between the CYP24A1 rs4809957 gene polymorphism and the histopathological grade of CRC patients at Prof. Dr. I G.N.G. Ngoerah Hospital in 2018-2019. Methods: This research was an observational analytic study with a cross-sectional design. This study used 34 samples of stored biological material from CRC tissue. CYP24A1 polymorphism (rs4809957) was identified using qPCR. Results: The results showed that the mean ± SD of the patient’s age was 61.09 ± 11.79 years. A total of 18 patients (52.9%) of the patient sample were male, and 16 patients (47.1%) were female. The histopathological grade of CRC in this study consisted of 30 samples (88.2%) of low grade and 4 (11.8%) of high grade. There were 21 patients (61.8%) who had a history of smoking. In addition, 20 patients (58.8%) had a history of consuming alcoholic beverages. This study’s CYP24A1 rs4809957 gene polymorphism comprised the AA+AG genotype in 23 samples (67.6%) and GG in 11 (32.4%). There was no significant association between the CYP24A1 rs4809957 gene polymorphism and the histopathological grade of CRC with a p-value = 0.580 and PR (95%CI) = 2.33 (0.83 – 19.42). Conclusion: This study concluded that there was no significant association between the CYP24A1 rs4809957 gene polymorphism and the histopathological grade of CRC patients.