The assessment of asbestos and carbon nanotubes induced genotoxic effects

Abstract

Event Abstract Back to Event The assessment of asbestos and carbon nanotubes induced genotoxic effects Deniz Öner1*, Manosij Ghosh1, Eveline Putzeys1, Lode Godderis1 and Peter Hoet1 1 KU Leuven, Environment and Health,Laboratory of Toxicology, Belgium Carbon nanotubes (CNT) are one of the most promising engineered nanomaterials. CNT are tubular fiber shaped engineered graphites and produced in two common types: single wall carbon nanotube (SWCNT) and multi wall carbon nanotube (MWCNT). Unfortunately, their unique properties, making CNT valuable in a large number of applications, can be problematic for their potential toxicity. Moreover, CNT share some physic-chemical properties with asbestos, a known carcinogen fiber if inhaled. In the current presentation, the genotoxicity of CNT in vitro will be discussed along with specific consideration concerning the validity of the FpG comet assay in CNT exposure. We have exposed MWCNT and SWCNT in human bronchial epithelial cell line (16HBE) and in human monocytic cell line (THP-1). FpG comet assay and micronucleus assay were performed in order to determine oxidative DNA strand breaks and aberrant micronuclei formation induced by CNTs. CNT can induce strand breaks and oxidative DNA damage. Both types of CNT induced a significant increase in % Tail DNA and Tail Moment in both cell types, whether or not in the presence of FpG. The micronuclei formation in the presence of cytochalasin B was apparent in all exposed 16HBE cultures, but not in THP-1 cultures. Without cytochalasin B, only MWCNT exposed 16HBE cells and the highest dose of SWCNT in THP-1 showed a significant increase in micronuclei. In conclusion we have observed both DNA damage and aberrant micronuclei formation in 16HBE cells. In THP-1 cells, although oxidative DNA damage and strand breaks occurred, aberrant micronuclei formation occurred in less extent. In all instances, interaction between CNT and assay system was avoided by adjusted protocols, and will be discussed during the presentation. Acknowledgements The work is financially supported by the “Stichting Tegen Kanker” (Agreement N° 2012-­‐218, Project N° 3M150270) Keywords: Comet Assay, Nanoparticles, Nanotubes, Carbon, Micronucleus Tests, oxidative DNA damage Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Oral Presentation Topic: BEMS satellite meeting Citation: Öner D, Ghosh M, Putzeys E, Godderis L and Hoet P (2015). The assessment of asbestos and carbon nanotubes induced genotoxic effects. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00075 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Aug 2015; Published Online: 25 Aug 2015. * Correspondence: Dr. Deniz Öner, KU Leuven, Environment and Health,Laboratory of Toxicology, Leuven, Belgium, Deniz.oner@med.kuleuven.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Deniz Öner Manosij Ghosh Eveline Putzeys Lode Godderis Peter Hoet Google Deniz Öner Manosij Ghosh Eveline Putzeys Lode Godderis Peter Hoet Google Scholar Deniz Öner Manosij Ghosh Eveline Putzeys Lode Godderis Peter Hoet PubMed Deniz Öner Manosij Ghosh Eveline Putzeys Lode Godderis Peter Hoet Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.