Abstract
The aim of this work was to study the value of the main allergen Asp n 3 of Aspergillus niger as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of A. niger as a contaminant and cause of spoilage of Mangifera indica. Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of A. niger was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region of the Asp n 3 encoding gene allowed us to identify the presence of the closely related fungal species Aspergillus fumigatus by detecting Asp n 3 homologous protein, which can be cross-reactive. The use of conserved segments of the Asp n 3 gene or its entire sequence allows us to detect phylogenetically closely related species within the Aspergilaceae family or to identify species-specific contaminating fungi.
Highlights
Several species belonging to the fungal genus Aspergillus are well known as plant pathogens that may cause damage in plants as well as spoil postharvest fruit, vegetables, and cereals, resulting in serious agriculture and economic losses (Perrone et al, 2007; Samson et al, 2010; Mahfooz et al, 2017)
The objective of this work was to study the value of the main allergen Asp n 3 of A. niger as a molecular marker of allergenicity and pathogenicity, with the potential to be used in the identification of A. niger as a contaminant and cause of spoilage of Mangifera indica
Inoculation with A. niger resulted in a depressed, circular, brown, soft area surrounding the point of insertion of the pathogen
Summary
Several species belonging to the fungal genus Aspergillus are well known as plant pathogens that may cause damage in plants as well as spoil postharvest fruit, vegetables, and cereals, resulting in serious agriculture and economic losses (Perrone et al, 2007; Samson et al, 2010; Mahfooz et al, 2017). Several authors report that the Aspergillus niger species complex can be responsible for the postharvest decay of different fresh fruits and some vegetables (Gautam et al, 2011; Sharma, 2012). Its extensive ubiquity in nature probably justifies why this species is one of the most common contaminating fungal genera recorded in various agricultural commodities, such as fruit, nuts, beans, cereals, and vegetables (Samson et al, 2010). On the other hand, selected A. niger strains are used in agriculture as promising beneficial microbes (Lubna et al, 2018; Pertile et al, 2021)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
