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Abstract
In this paper, we show the technical art of ICSI at our laboratory. We show the basic principal of ICSI on mammalian eggs, especially mouse and bovine ICSI. The use of Piezo-micromanipulator is useful for mouse and bovine ICSI, and how to use of piezo-electric actuator is shown carefully in this paper. The important point of mouse ICSI is the survival of eggs after sperm injection. On the other hand, in bovine ICSI, the accurate sperm injection and the less injection volume by using the piezo-electric actuator are important for successful bovine ICSI.
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