The art of chromosome dynamics: an interview with Jane Skok.

Abstract

In this interview, Professor Jane Skok speaks with Storm Johnson, commissioning editor for Epigenomics, on her work to date in the field of chromosome architecture and regulatory elements. Jane Skok's lab uses sophisticated microscopic techniques to visualize recombination in individual cells, tracing the dynamic changes in chromosome architecture and nuclear location at different stages of this complex process. This line of research unites two lifelong passions: science and art. After completing her PhD in immunology and genetics at the Imperial Cancer Research Fund in Lincoln's Inn Fields, DrSkok took 12 years off and pursued training in art while caring for her young children. She then returned to science, joining David Gray's lab at Imperial College London as a postdoctoral fellow to study B cell biology and acquired expertise in Mandy Fisher's lab to understand how nuclear organization of the antigen receptor genes regulate V(D)J recombination and allelic exclusion. DrSkok continued to pursue these questions in her own lab at University College London and elucidated the roles of Pax5, locus contractionand nuclear subcompartmentalization in maintaining allelic exclusion. In 2006, DrSkok was recruited to New York University School of Medicine, where her lab has revealed the activities of several signaling factors in guiding B cell development and they made the surprising discovery that the RAG proteins and the DNA damage response factor ATMhelp ensure allelic exclusion at the immunoglobulin gene loci. More recently, those atthe Skok lab have turned their attention to understanding how localized and long-range chromatin contacts impact gene regulation in health and disease settings.