The Arf GEF GBF1 integrates inputs from its product, Arf4·GTP, and the Golgi influx of sensory membrane cargo to activate Arf4 in ciliary trafficking

D Deretic,J Wang

doi:10.1186/2046-2530-4-s1-p24

Abstract

Objective Primary cilia in biology and disease are of considerable importance, however, molecular mechanisms for specific targeting of sensory receptors to primary cilia are still unknown. In the present study we wanted to determine if the small GTPase Arf4 is activated by the Arf guanine nucleotide exchange factor (GEF) GBF1 in ciliary trafficking, and examine the role of the ciliary cargo, rhodopsin, in the activation of Arf4.

Highlights

ObjectivePrimary cilia in biology and disease are of considerable importance, molecular mechanisms for specific targeting of sensory receptors to primary cilia are still unknown
Our findings suggest that GBF1 acts both as a guanine nucleotide exchange factor (GEF) and an effector of Arf4
The Arf GEF GBF1 co-localized with the Rab6 GTPase at the photoreceptor trans-Golgi

Summary

Objective

Primary cilia in biology and disease are of considerable importance, molecular mechanisms for specific targeting of sensory receptors to primary cilia are still unknown. In the present study we wanted to determine if the small GTPase Arf is activated by the Arf guanine nucleotide exchange factor (GEF) GBF1 in ciliary trafficking, and examine the role of the ciliary cargo, rhodopsin, in the activation of Arf

Methods

Conclusions

Results