Abstract
CDPKs (Ca2+-Dependent Protein Kinases) are very important regulators in plant response to abiotic stress. The molecular regulatory mechanism of CDPKs involved in salt stress tolerance remains unclear, although some CDPKs have been identified in salt-stress signaling. Here, we investigated the function of an Arabidopsis CDPK, CPK12, in salt-stress signaling. The CPK12-RNA interference (RNAi) mutant was much more sensitive to salt stress than the wild-type plant GL1 in terms of seedling growth. Under NaCl treatment, Na+ levels in the roots of CPK12-RNAi plants increased and were higher than levels in GL1 plants. In addition, the level of salt-elicited H2O2 production was higher in CPK12-RNAi mutants than in wild-type GL1 plants after NaCl treatment. Collectively, our results suggest that CPK12 is required for plant adaptation to salt stress.
Highlights
Saline soil cannot be used for agriculture and forestry production [1], and soil salinity is a major abiotic stress for plants worldwide [2,3]
We examined whether CPK12 affects seedling growth under salt stress
Our results suggest that the function of CPK12 may not be redundant for CPK27, because independent down-regulation of CPK12 or CPK27 can change the responses of Arabidopsis seedlings to salt stress
Summary
Saline soil cannot be used for agriculture and forestry production [1], and soil salinity is a major abiotic stress for plants worldwide [2,3]. The expression of CPK27 is induced by NaCl, and the cpk mutant is much more sensitive to salt stress than wild-type plants in terms of seed germination and post-germination seedling growth [54]. Heterologous overexpression of ACPK1 in Arabidopsis promotes significant plant growth and enhances ABA sensitivity in seed germination, early seedling growth, and stomatal movement, providing evidence that ACPK1 is involved in ABA signal transduction as a positive regulator [63]. InInt.t.JJ..MMooll..SSccii..22001188,, 1199,, 4x0F6O2 R PEER REVIEW Int. J. CPK12 and other CDPK members constitute a complicated regulation network, which functions in plant adaptation to salt and drought stresses. CDPKs constitute a network, which at the whole-plants level in roots and shoots, uses interaction and phosphorylation to regulate ion transporters or channels to improve plant tolerance to drought or salt stress over the short term
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