Abstract
Natural genetic variation in photosynthesis is strictly associated with the remarkable adaptive plasticity observed amongst Arabidopsis thaliana accessions derived from environmentally distinct regions. Exploration of the characteristic features of the photosynthetic machinery could reveal the regulatory mechanisms underlying those traits. In this study, we performed a detailed characterisation and comparison of photosynthesis performance and spectral properties of the photosynthetic apparatus in the following selected Arabidopsis thaliana accessions commonly used in laboratories as background lines: Col-0, Col-1, Col-2, Col-8, Ler-0, and Ws-2. The main focus was to distinguish the characteristic disparities for every accession in photosynthetic efficiency that could be accountable for their remarkable plasticity to adapt. The biophysical and biochemical analysis of the thylakoid membranes in control conditions revealed differences in lipid-to-protein contribution, Chlorophyll-to-Carotenoid ratio (Chl/Car), and xanthophyll cycle pigment distribution among accessions. We presented that such changes led to disparities in the arrangement of the Chlorophyll-Protein complexes, the PSI/PSII ratio, and the lateral mobility of the thylakoid membrane, with the most significant aberrations detected in the Ler-0 and Ws-2 accessions. We concluded that selecting an accession suitable for specific research on the photosynthetic process is essential for optimising the experiment.
Highlights
The main objective of this work was to elucidate the changes in the photosynthetic apparatus between six Arabidopsis ecotypes: Col-0 (N1092), Col-1(N3176), Col-2 (N907), Col-8 (N60000), Ws-2 (N22659), and Ler-0 (NW20)
Obtained data revealed that the efficiency of Photosystem II (PSII) (Fv/Fm) in all analysed plants was above the value of 0.8—equal to the optimal level determined earlier for non-stressed wild-type plants [44,45] with the highest value detected in Col-0 and Col-1 (Figure 1D, Table A1)
Our results revealed that the A/L was similar in Col-0, Col-1, and Col-8, whereas the ratio value was lower in Ler-0, Ws-2, and Col-2
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. The inbreeding annual plant Arabidopsis thaliana inhabits a wide range of climates across its native Eurasian range [1,2]. Arabidopsis specimens collected in nature that represent homozygous genotypes are called ecotypes. Arabidopsis ecotypes are known for their remarkable adaptability and a vast diversity of forms. The first Arabidopsis accessions subjected to experiments were collected near Limburg in Germany by a botanist Friedrich Laibach at the beginning of the 20th century [3]. The extensive seed collection (ecotypes and X-ray induced mutants) established by Professor Laibach served as the basis for the Arabidopsis Information Service (AIS) seed bank and eventually, with the support and interest of other scientists, for modern stock centres such as the NASC
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