Abstract
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45°C. CRISPR/LbCpf1 (LbCas12a) is a temperature-sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soybean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high temperature-resistant crop. In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. Moreover, we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding.
Highlights
Citation for published item: Li, Bo and Liang, Sijia and Alariqi, Muna and Wang, Fuqiu and Wang, Guanying and Wang, Qiongqiong and Xu, Zhongping and Yu, Lu and Zafar, Muhammad Naeem and Sun, Lin and Si, Huan and Yuan, Dao jun and Guo, Weifeng and Wang, Yanqin and Lindsey, Keith and Zhang, Xianlong and Jin, Shuangxia (2020) 'The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypolfree cotton.', Plant biotechnology journal., 19 (2)
The temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high temperature-resistant crop
In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency
Summary
Citation for published item: Li, Bo and Liang, Sijia and Alariqi, Muna and Wang, Fuqiu and Wang, Guanying and Wang, Qiongqiong and Xu, Zhongping and Yu, Lu and Zafar, Muhammad Naeem and Sun, Lin and Si, Huan and Yuan, Dao jun and Guo, Weifeng and Wang, Yanqin and Lindsey, Keith and Zhang, Xianlong and Jin, Shuangxia (2020) 'The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypolfree cotton.', Plant biotechnology journal., 19 (2). The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol-free cotton
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