Abstract
Abstract RNA interference (RNAi) technology is a gene-silencing tool with high specificity and efficiency for gene function studies. The use of RNAi libraries, which is based on this technology, promotes the significance of RNAi in gene silencing, and furthermore it provides a basis for the large-scale gene function analyses and high-throughput gene scan studies. This paper reviews the several types of RNAi library preparation, their respective construction approaches and some critical issues during RNAi library construction.
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