Abstract
The derivatization of peptides for mass spectrometric sequencing involves treatment with trimethylanilinium hydroxide and methyl trifluoroacetate, followed by pyrolytic conversion to the corresponding TFA-peptide esters in the solid probe tip of a mass spectrometer. The mass spectra of TFA- peptide esters obtained by the pyrolysis procedure are essentially identical to reference spectra obtained from TFA derivatives prepared by conventional esterification procedures.
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