Abstract
Serotonin (5HT) concentration in plasma is usually determined by the method of Crawford and Rudd (1). This method is based upon the fluorescence of serotonin in strongly acidic solutions. Since the level of serotonin in human plasma is low, this method is technically difficult. Recently, a much more sensitive method, the measurement of 5HT using the fluoresence of the ninhydrin (5HT) reaction, has been employed for the assay of tissue serotonin (2–4). However, a search of the literature indicates that this method has not been applied to determination of plasma serotonin. We have now found the optimum conditions for application of the ninhydrin reaction to the problem of plasma serotonin assay. Our method appears to be both specific and sensitive for the determination of human blood or plasma serotonin.
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