The Application of Next Generation Sequencing in Forensic DNA Database

Abstract

With the developing of standardized forensic DNA profiling and information technology, the forensic DNA database has achieved a big success. By managing、searching and sharing through DNA database, the molecular biology and genetics play a crucial role in individual identification, in the meantime, the DNA database also promote the developing of large-scale forensic DNA testing. In recent years, the arising of second generation sequencing(SGS) has substantially changed the way of genetics research, and varies of platform、kit and system has been developed continuously, many of which has been applied in forensic laboratories. So for,the China National DNA Database has expanded to be the largest one in the world, therefore, the SGS needs to incorporated with the DNA database rather than instead of it. So, the compatibility of SGS-based data with massive PCR-CE data in the database is now becoming a critical problem.in this article, the China National DNA Database is introduced, the advantages of SGS and its possible role in the forensic DNA profiling and databasing are elaborated, and the consistency in data storage and the compatibility in comparing of the SGS-based data within the present DNA database are also discussed.