Abstract
The glucosinolate-myrosinase system which widely existed in cruciferous plants can protect plants from insects because glucosinolates will breakdown to isothiocyanates by endogenous myrosinase after plant cells are crushed. In recent years, isothiocyanates have been found to have good anti-cancer activity. Sulforaphene isolated from radish is one isothiocyanate that has the highest anticancer activity. However, when endogenous myrosinase was used to catalyze glucosinolates, the miscellaneous enzyme in the raw material would cause most of the substrate breakdown to by-product nitriles, which not only lead to a significant reduction in yield of sulforaphene, but also increased the separation difficulty. Therefore, we have developed a process for the production of sulforaphene using purified and immobilized myrosinase, which has resulted in a steady increase of the substrate conversion rate from 10% to 30% to more than 80%. It not only increased yield of sulforaphane but also increased the range of radish to choose from.
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