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Abstract
The possibility of application of five different metallochromic derivatives of Thymol Blue (I–V) to the spectrophotometric determination of micro amounts of copper has been studied. The derivative of proline (I), applied in a solution buffered with hexamethylenetetramine and hydrochloric acid, has proved best for this purpose. Absorbance of its copper complex shows only insignificant changes in the pH range from 4.9 to 5.4 and it conforms to Beer's law up to 25 μg of copper II at 595 mμ. Larger quantities of iron II, cobalt II, nickel II, zinc II, lead II, uranium II and beryllium II interfere.
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