Abstract
Formula granule (FG), as an improved preparation form of traditional liquid dedoction, is of increasing interest and hence it is necessary to develop facile analytical or discrimination methods. Based on the established 3-Stage IR method, a new external disturbance of lab environment (LE) in which room temperature (RT) is 25-30°C and humidity is 25-30%, is introduced to test its applicability in discrimination of Danggui formula granules from different companies in which the LE is used to affect the prepared KBr pellet to produce enough variations for the 3S-IR method. Firstly, the 2D-spectra and pattern recognition algorithm of principal component analysis (PCA) and hierarchical cluster analysis (HCA) are used to select the analytical basis for 2D-COS IR. Secondly, LE-based 2D-COS IR is used for the discrimination. The newly-established LE-based IR can avoid the complex parts of programmed temperature and sampling in IR instrument, and achieve the discrimination of different Danggui Formula Granules.
Highlights
Angelicae Sinensis Radix is officially recorded as traditional Chinese medicine (TCM) to possess the function of enriching blood, activating blood circulation, regulating menstruation and amenorrhoea, relieving pain and relaxing bowels [1, 2]
In the range of 1800-800 cm-1, the peaks become complex: for instance, the 1650 cm-1 is attributed to C=O stretching vibration, the 1450 cm-1 is attributed to phenyl ring, the 1100-900 cm-1 is attributed to C-O stretching vibration
lab environment (LE) is applied as the external disturbance in the 3S-IR method
Summary
Angelicae Sinensis Radix (called Danggui in Chinese, abbreviated as DG hereinafter) is officially recorded as traditional Chinese medicine (TCM) to possess the function of enriching blood, activating blood circulation, regulating menstruation and amenorrhoea, relieving pain and relaxing bowels [1, 2]. Bi Xiaodong et al.: The Application of Lab Environment as the External Disturbance in the 3-Stage IR Method for the Facile Discrimination of Danggui Formula Granules. Componential analysis, used in the analysis of FGs, usually includes two kinds: (1) liquid chromatography (LC)-based methods [11,12,13,14] for providing much information of detailed compositions, namely micro-fingerprint. Since IR-based methods can provide both holistic feature and detailed information at the same time in rapid differentiation of TCM and TCM preparations, it is necessary to explore new ways to implement the external disturbance for wide and convenient application of 3S-IR. Using the DG-FGs of different pharmaceutical companies as the test samples, this LE was proved to be an effective external disturbance, by which the rapid discrimination of the chosen DG-FGs was achieved on the basis of the 3S-IR method
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