Abstract
We describe the application of wide-field frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM) to imaging in microfluidic devices. FLIM is performed using low cost, intensity modulated Light Emitting Diodes (LEDs) for illumination. The use of lifetime imaging for quantitative analysis within such devices is demonstrated by mapping the molecular diffusion of iodide ions across a microchannel.
Highlights
Microfluidic devices represent an excellent platform to implement a large number of chemical processing and sensing applications [1,2,3,4,5,6,7]
A key factor in the usefulness of microfluidics is the availability of matching online diagnostic systems that permit as much physical and chemical information to be sampled as possible
Optical techniques are very powerful in this context and in the past techniques have focused on fluorescence microscopy [8], light microscopy [9], confocal laser scanning microscopy [10], and optical coherence tomography [11]
Summary
Microfluidic devices represent an excellent platform to implement a large number of chemical processing and sensing applications [1,2,3,4,5,6,7]. A key factor in the usefulness of microfluidics is the availability of matching online diagnostic systems that permit as much physical and chemical information to be sampled as possible Such analytical techniques require species selectivity, sensitivity, and a high spatial and temporal resolution to be useful for online process monitoring, sensing applications, and product characterisation inside microfluidic channels. Spectral overlap may cause ambiguity preventing a discrimination of the different components In such situations the fluorescence lifetime of a sample may give additional or superior information
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