The application of different Bacillus subtilis contained formula as bio fungicide tablet to control Ganoderma boninense in oil palm nurseries

Abstract

This study aimed to determine effect of different Bacillus sp. endophytic contained formula as bio fungicide tablet to control Ganoderma boninense in oil palm nursery. A Complete Randomized Design, 5 treatments and 4 replicates were used. The treatments were different formula namely: F0 = Bacillus sp. endophytic, F1 = Bacillus sp. endophytic + sago starch + peat + tapioca flour, F2 = Bacillus sp. endophytic + corncobs flour + sago dregs + tapioca flour, F3 = Bacillus sp. endophytic + solid + talk + tapioca flour, F4 = Bacillus sp. endophytic + pineapple skin flour + zeolite + tapioca flour. Data were analyzed using ANOVA at a = 5%. The results showed that all formulas were able to slow down incubation period and reduce disease intensity. However, the increase of seedlings height, number of leaves, increase in tuber diameter, root volume, root canopy ratio and dry weight of roots is not affected. F3 was found as best formula due to able control G. boninense by slowing down disease incubation period and reduce its intensity to 0% within 140 days. That formula also shows better in increasing seedling height, number of leaves, diameter of stem, root volume, and weight dried root.