Abstract
Monitoring of all critical process parameters in bioprocess engineering is essential. Sensors have been previously developed for specific parameters such as on-line temperature, pH or stirring control and data logging. However, biomass monitoring needs further development. All current non-invasive technology, such as Near Infra-Red, is limited on biomass measurement of animal and insect cells. Biomass monitoring of industrial bioprocesses of filamentous microorganisms still requires sample removal from the vessel, which could potentially compromise sterility. This study has focused on the application of a non-invasive optical sensor in the on-line monitoring of the biomass of the filamentous microorganism Streptomyces coelicolor A3 (2). Raw output data from the biomass monitor were directly compared to data from the sensors measuring dissolved oxygen levels and off gas evolution and the results successfully demonstrate that the optical sensor is sensitive in identifying different levels of biomass. Therefore, it is possible to use the simple output data to provide real time information on biomass levels of filamentous microorganisms, a very powerful tool in bioprocess engineering.
Highlights
Real time monitoring and precise control of all critical process parameters (CPPs) belong to the main focuses of bioprocess engineering [1]
Near infra-red spectroscopy (NIRS) is commonly practiced for growth monitoring through analyte concentration profiles, such as glucose, but is application is limited to dispersed cell growth [3]
In this study we focused on the use of a new non-invasive optical sensor (BugEye® 100) for the real time monitoring of biomass of the filamentous microorganism Streptomyces coelicolor A3 (2)
Summary
Real time monitoring and precise control of all critical process parameters (CPPs) belong to the main focuses of bioprocess engineering [1]. This will ensure high productivity, robustness and reproducibility. NIRS technology has been previously employed to measure analytes in the filamentous fermentation of Streptomyces fradiae, such as methyl oleate, glucose, glutamate and ammonium, but not biomass concentrations [6]. In this study we focused on the use of a new non-invasive optical sensor (BugEye® 100) for the real time monitoring of biomass of the filamentous microorganism Streptomyces coelicolor A3 (2). S. coelicolor is the model actinomycete and its genome was sequenced in 2002 [7]
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