Abstract
Excised roots of six potato selections were challenged with egg inoculum of fourMeloidogyne species:M. chitwoodi, M. javanica, M. incognita, andM. hapla. The dual cultures were established under aseptic conditions in 60 × 15 mm petri plates on a defined agar medium. Egg hatch occurred within one week and galling within four weeks at incubation temperature of 24°C. All six potato selections, Russet Burbank, White Rose, PAS 3065,S. sparsipilum 365343.A19-12,S. sparsipilum ×S. phureja 856D68-1, and NDTX8731-1R supported reproduction of all fourMdoidogyne species. Corresponding greenhouse tests were run with eachin vitro experiment to establish the efficacy of this screening technique compared to greenhouse methods. Results from the greenhouse assays had less variation within cultivar treatment than did the results from thein vitro assays. Thein vitro assay, however, may be conducted with less labor, time, and greenhouse facility management.
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